Breast Cancer Research Lab — Dr. Cecelia Speyer
For the past five years, my collaborator and I have identified and established a role for the metabotropic glutamate receptor-1 (mGluR1) in breast cancer. Our specific focus includes i) determining the mechanism and novel pathways by which mGluR1 regulates the angiogenic process in endothelial cells ii) examining the molecular pathways by which mGluR1 mediates growth and metastatic properties of breast cancer cells iii) elucidating the mGluR1-dependent and independent role of the drug, Riluzole, in regulating breast tumor progression iv) examining the anti-inflammatory properties of mGluR1 and Riluzole within the tumor microenvironment.
My past research focus has been in the area of inflammation where I discovered the expression of novel chemokine receptors on neutrophils in sepsis. These findings have shed light on the concept of neutrophil subsets in various chronic inflammatory diseases such as sepsis, autoimmune diseases, and cancer. Currently, my laboratory’s focus is expanding into the area of tumor inflammation where we are examining the role and function of the various PMN subsets, their expression of various receptors (CC chemokine, mGluR1), and the role of the receptors themselves within the tumor microenvironment in the hopes of identifying targets for the therapeutic treatment of breast cancer.
Cecilia Speyer, Ph.D.
Principal Investigator, Breast Cancer Research Lab
Title: Assistant Professor, Department of Surgery, Wayne State University School of Medicine.
Office Address: Elliman Building Room 1221, 4100 John R., Detroit, MI, 48021
Phone: 1-313-576-8343 (Office), 1-313-576-8997 (Lab), 1-313-576-9322 (Fax)
Specialty: immunology and physiology
Research Interest: metabotropic glutamate receptor-1 (mGluR1) in breast cancer
Ph.D.: Wayne State University School of Medicine, Detroit, MI (Physiology and Immunology)
M.S.: Wayne State University School of Medicine, Detroit, MI (Basic Medical Sciences)
B.S.: Oakland University, Rochester, MI (Biochemistry)
ASC in Science: Oakland Community College, Royal Oak, MI
B.S., UofM – Dearborn, MI, U.S.A.
Fall 2011 to present
Undergraduate student at UofM – Dearborn, MI, U.S.A.
Winter 2012 to present
Undergraduate student at UofM – Dearborn, MI, U.S.A.
Spring 2012 to present
B.S. of Arts and History
B.S. of Science (Biochemistry)
Winter 2013 to present
Speyer, C.L., Hachem, A.H., Assi, A.A., Johnson, J.S., DeVries, J.A., and Gorski, D.H. Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer. PLOS ONE (submitted)
Banda, M., Speyer, C.L., Semma, S.N., Kingsley, O., Kim, H.J., Kounalaskis, N., Torres, K.E., Barnard, N.J., Sloane, B.F., Miller, F.R., Goydos, J.S., and Gorski, D.H. Metabotropic glutamate receptor-1 signaling is oncogenic in triple negative breast cancer. PLOS ONE (submitted)
Speyer, C.L., Smith, J.S., Banda, M., Mekani, T., Gorski, D.H. Metabotropic Glutamate receptor-1: A potential therapeutic target in triple negative breast cancer. Breast Cancer Res Treat, 132, 565-573, 2012.
Speyer, C.L. and Ward, P.A. Role of Endothelial Chemokines and their Receptors during Inflammation. J Invest Surg, 24(1), 18-27, 2011.
Chen, Y., Banda, M., Speyer, C.L., Smith, J.S., Rabson, A., and Gorski, D.H. Regulation of the expression and activity of the antiangiogenic homeobox gene GAX/MEOX2 by ZEB2 and microRNA-221. Mol Cell Biol, 20(15), 3902-13, 2010.
Gao, H., Neff, TA, Guo, R., Speyer, CL, Sarma, JV, Tomlins, S, Man, Y, Riedemann, NC, Hoesel, LM, Younkin, E, Zetoune, FS, and Ward, PA. Evidence for a functional role of the second C5a receptor C5L2. FASEB J, 19(8), 1003-5, 2005
Neff, T.A., Guo, R.F., Neff, S.B., Sarma, J.V., Speyer, C.L., Gao, H., Bernacki, K.D., Huber-Lang, M., McGuire, S., Hoesel, M., Riedemann, N.C., Beck-Schimmer, B., Zetoune, F.S., and Ward, P.A. Relationship of Acute Lung Inflammatory Injury to Fas/FasL System. Am J Path, 166, 685-694, 2005
Speyer, C.L., Rancilio, N.J., McClintock, S.D., *Crawford, J.D., Gao, H., Sarma, J.V., and Ward, P.A. Regulatory effects of estrogen on acute lung inflammation in mice. Am J Physiol Cell Physiol, 288, C881-890, 2005
Speyer, C.L., Gao, H., Rancilio, N.J., Neff, T.A., Huffnagle, G.B., Sarma, J.V., and Ward, P.A. Novel chemokine responsiveness and mobilization of neutrophils during sepsis. Am J Path, 165, 1-10, 2005
Warner, R.L., Winter, H.C., Speyer, C.L., Varani, J., Goldstein, I.J., Murphy, H.S., and Johnson, K.J. Marasmius oreades lectin induces renal thrombotic microangiopathic lesions. Exp. Molec. Pathol, 77, 77-84, 2004
Gao, H., Guo, R., Speyer, C.L., Reuben, J., Neff, T.A., Hoesel, L.M., Riedemann, N.C., McClintock, S.D., Sarma, J.A., Rooijen, N.V., Zetoune, F.S., and Ward, P.A. Stat3 activation in acute lung injury. J Immun, 172 (12), 7703-12, 2004
Laudes, I.J., Guo, R.F., Riedemann, N.C., Speyer, C., Craig, R., Sarma, J.V., and Ward, P.A. Disturbed homeostasis of lung intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 during sepsis. Am J Pathol. 164, 1435-45, 2004
Riedemann, N.C., Guo, R.F., Hollmann, T.J., Gao, H., Neff, T.A., Reuben, J.S., Speyer, C.L., Sarma, J.V., Wetsel, R.A., Zetoune, F.S., and Ward, P.A. Regulatory role of C5a in LPS-induced IL-6 production by neutrophils during sepsis. FASEB J. 18, 370-2, 2004
Speyer, C.L., Neff, T.A., Warner, R.L., Guo, R.F., Sarma, J.V., Riedemann, N.C., Murphy, M.E., Murphy, H.S. and Ward, P.A. Regulatory effects of iNOS on acute lung inflammatory responses in mice. Am J Pathol. 163, 2319-28, 2003
Riedemann, N.C., Guo, R.F., Bernacki, K.D., Reuben, J.S., Laudes, I.J., Neff, T.A., Gao, H., Speyer, C.L., Sarma, V.J., Zetoune, F.S., and Ward, P.A. Regulation by C5a of neutrophil activation during sepsis. Immunity. 19, 193-202, 2003
Herskovic, J.J., Speyer, C.L., Simples, J.E., Steffes, C.P. and Ram, J.L. Lipopolysaccharide (LPS) enhancement of outward current in lung pericytes. Lung 180, 215-220, 2002
Laudes, I.J., Chu, J.C., Huber-Lang, M., Guo, R.F., Riedemann, N.C., Sarma, J.V., Mahdi, F., Murphy, H.S., Speyer, C., Lu, K.T., Lambris, J.D., Zetoune, F.S., and Ward, P.A. Expression and function of C5a receptor in mouse microvascular endothelial cells. J Immun. 169, 5962-5970, 2002
Riedemann, N.C., Guo, R.F., Sarma, V.J., Laudes, I.J., Huber-Lang, M., Warner, R.L., Albrecht, E.A., Speyer, C.L., and Ward, P.A. Expression and function of the C5a receptor (C5aR) in rat alveolar epithelial cells. J Immun. 168, 1919-25, 2002
Speyer, C.L., Steffes, C.P., Tyburski, J.G., Homan, R., and Ram, J.L. Lipopolysaccharide-induced secretory phospholipase A2 activity in pericytes: a possible mechanism for mediating relaxation. Microvasc Res. 63, 239-242, 2002
Kerkar, S., Speyer C., Tyburski, J. and Steffes, C. Reactive oxygen metabolites induce a biphasic contractile response in microvascular lung pericytes. J Trauma. 51, 440-5, 2001
Dente, C.J., Steffes, C.P., Speyer C.L., and Tyburski, J.G. Pericytes augment the capillary barrier in in-vitro cocultures. J Surg Res. 97, 85-91, 2001
Speyer, C.L. Regulation of LPS-induced relaxation in the rat lung pericyte. Dissertation, Wayne State University, Detroit, MI, 2000
Speyer, C.L., Steffes, C.P., Tyburski, J.G., and Ram, J.L. Lipopolysaccharide induces relaxation in lung pericytes by an iNOS-independent mechanism. Am J Physiol: Lung Cell Mol Physiol. 278, L880-L887, 2000
Speyer, C.L., Steffes, C.P., and Ram, J.L. Effects of vasoactive mediators on the rat lung pericyte: quantitative analysis of contraction on collagen lattice matrices. Microvasc Res. 57, 134-143, 1999
Purchase, C.L., II, White, A.D., Anderson, M.K., Bocan, T.M.A., Bousley, R.F., Hamelehle, K.L., Homan, R., Krause, B.R., Lee, P., Bak-Mueller, S., Speyer, C., Stanfield, R.L. and Reindel, J.F. Tetrazole-substituted ureas as inhibitors of acyl-CoA:cholesterol O- acyltransferase (ACAT). A novel preparation of ureas from weakly nucleophilic amines. Bioorganic Med. Chem. Lett. 6, 1753-1758, 1996
Lee, H.T., Sliskovic, D.R., Picard, J.A., Roth, B.D., Wierenga, W., Hicks, J.L., Bousley, R.F., Hamelehle, K.L., Homan, R., Speyer, C., Stanfield, R.L. and Krause, B.R. Inhibitors of acyl-CoA:cholesterol O-acyltransferase (ACAT) as hypocholesterolemic agents. CI-1011: an acyl sulfamate with unique cholesterol-lowering activity in animals fed noncholesterol-supplemented diets. J. Med. Chem. 39, 5031-5034, 1996
Invited speaker for the Breast Cancer Biology & Proteases and Cancer Joint Retreat Program (December 14, 2010)
Invited speaker for the Biological Sciences Departmental Seminar Series at University of Michigan – Flint (June 29, 2010)
Invited lecturer (gastrointestinal immunology) for the Department of Physiology at Wayne State University School of Medicine (April 2007 to 2008)
Invited speaker for the 2005 Physiology Department Graduate Student Day Program at Wayne State University School of Medicine
Invited presenter for the Northville Public School’s Career Day Seminar Series 2002
Dissertation presentation and defense in the Physiology Department at Wayne State University School of Medicine 2000