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Meet Our Faculty

Bala K Kolli, PhD

Adjunct Assistant Professor
Office Address:
3333 Green Bay Road
Department of Surgery
North Chicago, 60064
Phone: 847-942-2419
Fax:

Administrative Assistant: Jamie Carson
Administrative Email: jcarson@med.wayne.edu

SPECIALTY: Microbiology
CLINICAL INTERESTS:

RESEARCH INTERESTS

  1. Biosimilars: Designing vectors for secreted antibody production in mammalian cells for treatment of various cancers. We have generated clones of cell lines that secrete several antibodies. We are at present pursuing production of these antibodies in bioreactors. We have standardized protocol for antibody purification. Once purified from bioreactor culture, we will characterize these antibodies for their effectiveness, both in vitro and in vivo in animal models for their subsequent use in human trials.
  1. Chimeric Antigen Receptor (CAR) mediated cancer treatment: Design and construction of vectors for CAR, successfully introduced into T and NK cells. These transfected cells show activity against cancer lines. The CAR vector design includes features that can be used to generate any CAR with minimal manipulation.
  1. Molecular parasitology: Various aspects of the human protozoan parasite, Leishmania. including evolution of these parasites and molecules that are involved in invasion of the host cells and evasion of human immune response. Recent focus was on photodynamic vaccination against leishmaniasis—molecular parasitology research particularly .

 

Education

EDUCATION

Graduate: School of Life Sciences, Jawaharlal Nehru University, New Delhi/Delhi, India — 1995
Phil.: School of Life Sciences, Jawaharlal Nehru University, New Delhi/Delhi, India — 1955
Sc. (Ag): Institute of Agricultural Sciences, Banaras Hindu University, Varanasi/Uttara Pradesh, India — 1985
Baccalaureate: Institute of Agricultural Sciences, Banaras Hindu University, Varanasi/Uttara Pradesh, India — 1983

POSTGRADUATE TRAINING
Advanced training in Photosynthesis and Algal Biotechnology, Sede-Boker, Israel — 1993-94
Research Associate, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India — 1995-98
Post-doctoral Fellow, Finch University of Health Sciences, North Chicago, IL 60064 — 1999-2001

Publications

PATENTS

  1. US patent No. 7,238,347 B2, July 3, 2007

Title: SUICIDAL MUTANT LEISHMANIA VACCINE

Applicant: Rosalind Franklin University of Medicine and Science

Inventors: Chang, KP, Kolli, BK, et al. 

  1. US patent No.  7,261,887 B2, August 28, 2007

Title: LEISHMANIA AS CARRIERS FOR THE DELIVERY OF PROTEINS AND PEPTIDES

Inventors: Chang, KP, Sassa, S, Sah, JF, Ito, H, Kolli, BK and Peterson, DA

Applicant: Rosalind Franklin University of Medicine and Science

  1. US Patent No. 62/323.064, April 10, 2017

Title: PHOTODYNAMIC INSECTICIDES

Applicant: Rosalind Franklin University of Medicine and Science

Inventors: Chang KP, Kolli BK, Shiao SH

  1. US Patent Application No. 15/970,540, November 8, 2018

Provisional

Title: IMMUNOTHERAPY OF CANINE LEISHMANIASIS

Applicant: Rosalind Franklin University of Medicine and Science

Inventors: Kwang-Poo Chang, Laura Manna, Raffaele Corso and Bala K. Kolli

PUBLICATIONS

Peer-Reviewed Publications

  1. Batchu RB, Gruzdyn OV, Kolli BK, Dachepalli R, Umar PS, Rai SK, Singh N, Tavva PS, Weaver DW, Gruber SA. IL-10 Signaling in the Tumor Microenvironment of Ovarian Cancer. Adv Exp Med Biol. 2021; 1290:51-65. 
  2. Sharma R, Viana SM, Ng DKP, Kolli BK, Chang KP, de Oliveira CI. Photodynamic inactivation of Leishmania braziliensis doubly sensitized with uroporphyrin and diamino-phthalocyanine activates effector functions of macrophages in vitro. Sci Rep. 2020; 10:17065.
  3. Batchu RB, Gruzdyn OV, Tavva PS, Kolli BK, Dachepalli R, Weaver DW, Gruber SA. Engraftment of mesothelin chimeric antigen receptor using a hybrid Sleeping Beauty/minicircle vector into NK-92MI cells for treatment of pancreatic cancer. Surgery. 2019; 166:503.
  4. Shiao SH, Weng SC, Luan L, Vicente MDGH, Jiang XJ, Ng DKP, Kolli BK, Chang KP. Novel phthalocyanines activated by dim light for mosquito larva- and cell-inactivation with inference for their potential as broad-spectrum photodynamic insecticides. PLoS One. 2019; 14:e0217355.
  5. Ertabaklar H, Çalışkan SÖ, Kolli B, Ertuğ S, Özbilgin A, Malatyalı E, Chang KP. [Transfection of Leishmania tropica with green fluorescent protein (gfp) gene and investigation of the in vitro drug effect]. Mikrobiyol Bul. 2019; 53:213.
  6. Viana SM, Celes FS, Ramirez L, Kolli B, Ng DKP, Chang KP, de Oliveira CI. Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis. Front Microbiol. 2018; 9:165. 
  7. Patil R, Kulshrestha A, Tikoo A, Fleetwood S, Katara G, Kolli B, Seibel W, Gilman-Sachs A, Patil SA, Beaman KD. Identification of Novel Bisbenzimidazole Derivatives as Anticancer Vacuolar (H⁺)-ATPase Inhibitors. Molecules. 2017; 22: pii: E1559.
  8. Mishra AK, Singh N, Agnihotri P, Mishra S, Singh SP, Kolli BK, Chang KP, Sahasrabuddhe AA, Siddiqi MI, Pratap JV. Discovery of novel inhibitors for Leishmania nucleoside diphosphate kinase (NDK) based on its structural and functional characterization. J Comput Aided Mol Des. 2017; 31:547. 
  9. Al-Qahtani A, Saad Alkahtani S, Kolli BK, Tripathi P, Dutta S, Al-Kahtane AA, Jiang XJ, Ng DKP, Chang KP. Amino-phthalocyanine-mediated photodynamic inactivation of Leishmania tropica. Antimicrob Ag Chemther. 2016; 60:2003. 
  10. Batchu RB, Gruzdyn OV, Moreno-Bost AM, Szmania S, Jayandharan G, Srivastava A, Kolli BK, . Weaver DW, van Rhee F and Gruber SA. Efficient Lysis of Epithelial Ovarian Cancer Cells by MAGE-A3-Induced Cytotoxic T Lymphocytes Using rAAV-6 Capsid Mutant Vector. Vaccine. 2014; 32:938.
  11. 11.  Katara GK, Jaiswal MK, Kulshrestha A, Kolli BK, Gilman-Sachs A, Beaman KD.  Tumor-associated vacuolar ATPase subunit promotes tumorigenic characteristics in macrophages. Oncogene. 2014; 33:5649.
  12. Dutta S, Chang C, Kolli BK, Sassa S, Yousef, Showe M, Showe L, Chang KP. Delta-aminolevulinate-induced host-parasite porphyric disparity for selective photolysis of transgenic Leishmania in the phagolysosomes of mononuclear phagocytes: A potential novel platform for vaccine delivery. Euk Cell. 2012; 11: 430. 
  13. Dutta S, Ongarora BG, Li H, Vicente MGH, Kolli BK, Chang KP. Intracellular targeting specificity of novel phthalocyanines assessed in a host-parasite model for developing potential photomedicine. PLoS ONE. 2011; 6:e20786.
  14. Kumari S, Samant M, Khare P, Misra P, Dutta S, Kolli BK, Sharma S, Chang KP, Dube A. Photodynamic vaccination of hamsters with inducible suicidal mutants of Leishmania amazonensis elicits T cell-transferable immunity against visceral leishmaniasis. Eup. J. Immunol. 2009; 38: 1-14.
  15. Varela M RE, Lorena Muñoz D, Robledo SM, Kolli BK, Dutta S, Chang KP, Muskus C. Leishmania (Viannia) panamensis: An in vitro assay using the expression of GFP for screening of antileishmanial drug. Exp Parasitol. 2009; 122:134.
  16. Kolli BK, Costa J, Chakrabarty A, Chang, KP. Leishmania release nucleoside diphosphate kinase to prevent ATP-dependent apoptosis of infected macrophages Mol Biochem Parasitol. 2008; 158:163.
  17. Mehta SR, Huang R, Yang M, Zhang XQ, Kolli B, Chang KP, Hoffman RM, Goto Y, Badaro R, Schooley RT. Real time in vivo gfp imaging in a murine leishmaniasis model: a new tool for leishmania vaccine and drug discovery. Clin. Vaccine Immunol. 2008; 15, 1764.
  18. Dutta S, Kolli BK, Tang A, Sassa S, Chang KP. Transgenic Leishmania model for delta-aminolevulinate-inducible monospecific uroporphyria: Cytolytic phototoxicity initiated by singlet oxygen-mediated inactivation of functional proteins and its ablation by evacuation of cytosolic uroporphyrin. Eukaryot. Cell. 2008; 7: 1146.
  19. Waki K, Dutta S, Ray D, Kolli BK, Kawazu SI, Lin CP, Chang KP. Transmembrane molecules for phylogenetic analysis of pathogenic protists: Leishmania-specific informative sites in hydrophilic loops of trans-ER N-acetylglucosamine-1-phosphate transferase. Eukaryotic Cell. 2007; 6: 198.
  20. Thiakaki M, Kolli BK, Chang KP, Soteriadou K. Down-regulation of gp63 level in Leishmania amazonensis promastigotes reduces their infectivity in BALB/c mice. Microbes Infect. 2007; 8:1455.
  21. Dutta S, Ray D, Kolli BK, Chang KP. Photosensitization of Leishmania amazonensis in both extracellular and intracellular stages with phthalocyanine chloride for photolysis in vitro. Antimicrobial Ag Chemother. 2005; 49: 4474.
  22. 22.Hajmova M, Chang KP., Kolli BK, Volf P. Down-regulation of gp63 in Leishmania amazonensis reduces its early development in Lutzomyia longipalpis. Microbes and Infection. 2004, 6: 646.
  23. Sah JF, Ito H, Kolli BK, Peterson DA, Sassa S, Chang KP. Genetic rescue of Leishmania deficiency in porphyrin biosynthesis creates mutants suitable for analysis of cellular events in uroporphyria and for photodynamic therapy. J Biol Chem. 2002; 277: 14902.
  24. Chen DQ, Kolli BK, Yadava N, Lu HG, Gilman-Sachs A, Peterson DA, Chang KP. Episomal expression of specific sense- and anti-sense mRNAs in Leishmania amazonensis; Modulation of gp63 level in promastigotes and their infection of macrophages in vitro. Inf. Immun. 2000; 68: 80.
  25. 25.Jha IB, Kolli BK, Sah JF, Garab G, Mohanty P. Low dose UV-B induced modification of chromophore conformation and it’s interaction with microenvironment in cyanobacterial phycobilisomes. Indian J Biochem Biophys. 2000; 37:486.
  26. 26.Kolli BK, Joshi MK, Vani B and Mohanty P. Structure-function correlation during the etioplast-chloroplast transition in cucumber cotyledonary leaves. Photosynthetica. 1999; 36: 199.
  27. 27.Kolli BK, Tiwari S, Mohanty P. Ultraviolet-B induced damage to photosystem II in intact filaments of Spirulina platensis. Z. naturforsch. 1998; 53c: 369.
  28. 28.Sah JF, Kolli BK, Mohanty P. Effects of ultraviolet-B radiation on phycobilisomes of Synechococcus PCC 7942. Biochem Mol Biol Int. 1998; 45: 245.
  29. 29.Kolli BK, Sah JF, Mohanty P. Ultraviolet-B induced alteration in energy transfer in phycobilisomes of Synechococcus PCC 7942. Indian J Exp Biol. 1996; 34: 1133.
  30. 30.Kolli BK, Sah JF, Mohanty P. Studies on electron donation to photosystem I sites by exogenous donors in Spirulina thylakoids. Indian J Biochem Biophys. 1996; 33:465.
  31. 31.Kolli BK, Varma A. Endoglucanase production by entrapped Bacillus thermoalkalophilus. World J Microbiol Biotechnol. 1990; 6:267.

Review Articles

  1. Chang KP, Kolli BK and Collaborators. Overview of Leishmaniasis with Special Emphasis on Kala-azar in South Asia. In: S.K. Singh (ed.), Neglected Tropical Diseases – South Asia. Springer International Publishing AG 2018.
  2. Chang KP, Kolli BK & New Light Group. New “light” for one-world approach toward safe and effective control of animal diseases and insect vectors from leishmaniac perspectives. Parasit Vectors. 2016; 9:396. 
  3. Varma A, Kolli BK, Paul J, Saxena S, Konig H. Lignocellulose degradation by microorganisms from termite hills and termite guts: A survey on the present state of art. FEMS Microbiol Rev. 1994; 15:9-28.

Published non-refereed Abstracts:

  1. Chang KP, Kolli BK, Ng DKP, Shiao SH, Manna L, Elliott RL, Fu GL (2018) Screening novel phthalocyanines for developing photodynamic-vaccination and –insecticides, in Light-based diagnosis and treatment of diseases, Dai T (ed) vol. 10479. Proc. Of SPIE- Int Soc Optic & Photonic, Feb. 26 – Mar 1, San Francisco.
  2. Chang KP, Kolli BK, Fan CK, Ng DNK, Wong CTT, Manna L, Corso R, Shih NY, Elliott RL, Jiang XP, Shiao SH, Fu GL (2018) “Progress toward development of photodynamic vaccination against infectious/malignant diseases and photodynamic mosquitocides”, Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 1047912 (8 February 2018); doi: 10.1117/12.2281437
  3. Chang KP, Kolli BK, Jiang XP, Elliott RL (2017) Photodynamic vaccination: Light inactivation of photo-sensitized Leishmania with singlet oxygen as safe and effective vaccines and vaccine carriers for immuno-prophylaxis and –therapy. WorldLeish 6, May 16-20, Toledo, Spain.
  4. Sharma R, Viana SM, Kolli BK, Chang, KP de Oliveira CI (2017). Delta-aminolevulinate-inducible uroporphyrinogensis in transgenic Leishmania braziliensis; a tool for photodynamic vaccination WorldLeish 6, May 16-20, Toledo, Spain.
  5. Viana SM, Celes FS, Laura Ramirez G., Kolli BK, Ng DKP, Chang KP, de Oliveira CI (2017). Photo-inactivation of Leishmania amazonensis as an immunoprophylaxis tool for Cutaneous Leishmaniasis.  Worldleish 6, May 16-20, Toledo, Spain.
  6. Manna L, Corso R, Kolli BK, Chang KP (2017). Immunotherapy of canine leishmaniasis by photodynamic vaccination.  WorldLeish6, May 16-20, Toledo, Spain.
  7. Mishra AK, Singh SP, Kolli BK, Bansal M, Chang KP, Pratap, JV (2013) Cloning, overexpression, purification, crystallization and data processing of Nucleoside Diphosphatase Kinase (NDK) from Leishmania amazonensis. #109, May 13-18, Worldleish 5, Recife, Brazil.
  8. Mehta SJ, Huang R, Yang M, Zhang XQ, Kolli B, Chang KP, Hoffman, Schooley RT. (2010) Evaluation of SLC11A1 role in host response to Leishmania amazonensis in vitro and in vivo. P. 40, #25, Ann Meet Soc Trop Med Hyg, Nov. 4, Atlanta, GA.
  9. Chang KP, Waki K, Kolli BK, Dutta S. (2009) Ambiguity of multiple DNA versus single-copy protein coding genes for Leishmania genotyping. # 10,  Feb. 2, “WorldLeish 4”, Lucknow, India.
  10. Chang KP, Kolli BK, Dutta S. (2009) Photodynamic vaccination with porphyric suicidal mutants against leishmaniasis, Feb. 4, #. 45, “WorldLeish 4”, Lucknow, India
  11. Kolli BK, Dutta S, Chang KP (2009) Leishmania releases nucleoside diphosphate kinase to prevent apoptosis of macrophages. Feb. 8, #323, “WorldLeish 4”, Lucknow, India
  12. Chang KP, Dutta S, Kolli BK (2009) Immunity to Leishmaniasis and Vaccination”, International Symposium on Leishmaniasis Vaccines, # 25, Mar. 9-11, Recife, Brazil
  13. Chang KP, Kolli BK, Waki K, Dutta S, Folkova D, Volf, P. (2006) Let the evils fight the devisls: Parasites against infectious diseases and cancer ? 4th Med. Congr. Pharm., Med. J. Sci. Tech. 2, P. 26.
  14. Kolli BK, Kostal J, Zaborina O, Chakraborty AM, Chang KP. (2002) Leishmania model for microbial virulence: Secretion of nucleoside diphosphate kinase prevents apoptosis of the host macrophages. 5th Ann. Meet. New & Re-emerging Dis., Urbana-Champaign University of Illinois, P. 25-26.
  15. Chang KP, Sah JF, Ito H, Kolli BK, Sassa S. (2000) Defects of trypanosomatid protozoa in heme biosynthesis: Porphyria of xenotransgenic Leishmania with mammalian heme pathway enzymes. Mill. Meet. Porphyrins Porphyrias 2000 P. 45.

Teaching

Advanced Course in Molecular Biology for graduate (Ph.D.) students. This course covers the latest advances in molecular biology.